Coding

Part:BBa_K3807000:Design

Designed by: Sarah Vorsselmans   Group: iGEM21_KU_Leuven   (2021-10-01)


enCas9-PolI3M-TBD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 6133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 6133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4168
    Illegal BamHI site found at 6393
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 6133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 6133
    Illegal NgoMIV site found at 1078
    Illegal NgoMIV site found at 5079
    Illegal AgeI site found at 4576
    Illegal AgeI site found at 6373
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1159


Design Notes

Taken from the original EvolvR paper (Halperin et al, 2018)

Source

Amplified from Addgene plasmid # 113077

References

Halperin et al, 2018